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Lymph nodes (axillary or popliteal) were collected in FACS buffer (1Ã PBS, 10% how to use kamagra 100mg oral jelly FCS, 2 mM EDTA) on ice cheap kamagra tablets. Single-cell suspensions were obtained by mechanical disruption through a 70-mm cell strainer (BD Biosciences). Erythrocytes were lysed cheap kamagra tablets with 1 ml ACK lysing buffer (Gibco). After incubation with 5 Âµg/ml anti-CD16/32 (rat mAb 2.4G2, mouse Fc block. BD Biosciences) for 15 min at 4Â°C, cells were stained for 30 min at 4Â°C.

When a biotinylated antibody was cheap kamagra tablets used, the cells were then incubated with a streptavidinâfluorophore conjugate for 20 min at 4Â°C. Flow cytometric analysis was performed on a BD LSRFortessa and Symphony. Antibodies used cheap kamagra tablets. From BD Biosciences, anti-IgM-e710 (R6-60.2, 550881), anti-IgD-BV786 (11-26c.2a, 563618), anti-CD95-PE-Cy7 (Jo2, 557653), and streptavidin-BV711 (563262). From BioLegend, anti-CD38-PB (90, 102719), anti-B220-BV605 (RA3-6B2, 103244), and live/dead marker Zombie NIR (423106).

And from eBiosciences, anti-T and -B cell activation antigen-e660 cheap kamagra tablets (GL7, 50-5902-82), anti-CD4-eF780 (RM4-5, 47-0042-82), anti-CD8-eF780 (53-6.7, 47-0081-82), anti-NK1.1-eF780 (PK136, 47-5941-82), anti-F4/80-eF780 (BM8, 47-4801-82), and anti-TM4-Core-biot (5 Âµg/ml) provided by Andrew T. McGuire and L. Stamatatos.Buffer used for FACS staining was a PBS, pH 7.2 cheap kamagra tablets (HyClone)âbased 1% BSA (Rockland Immunochemicals) 1 mM EDTA (Teknova) solution. Fc block was made by diluting heat-inactivated human AB serum (Valley Biomedical) to a 20% solution in FACS buffer. CD163 (GHI/61), HLA-DR (L243), CD40 (5C3), PD-L1 (29E.2A3), CD80 (2D10), CD14 (63D3), CD3 (OKT3), CD45RO (UCHL1), CD19 (4G7), CD56 (5.1H11), CD11b (M1/70), streptavidin-PE (405204), anti-mouse IgM PE (RMM-1), and anti-His PE (J095G46) FACS antibodies were all purchased from BioLegend.

Sdc2 antibody (305515) was purchased from R&D Systems cheap kamagra tablets. Anti-HS antibody 10E4 (F58-10E4) was purchased from AMSBIO. CITE-seq mAbs (TotalSeq B) included CD80 (2D10), CD274 (29E.2A3), CD3 (UCHT1), CD19 (HIB19), CD45RA (HI100), CD4 (RPA-T4), CD8 (RPA-T8), CD14 (M5E2), CD16 (3G8), CD56 cheap kamagra tablets (QA17A16), CD335 (9E2), CD62L (DREG-56), CD197 (G043H7), HLA-DR (L243), CD11b (ICRF44), and CD45RO (UCHL1. Table S4). Streptavidin-HRP (016-030-084) was purchased from Jackson ImmunoResearch Laboratories.

The ELISA Substrate Reagent Pack (DY999), ELISA cheap kamagra tablets plate-coating buffer (DY006), reagent diluent concentrate 2 (DY995), and Stop Solution (DY994) were purchased from R&D Systems. Anti-VISTA mAb1 and mAb2 were generated using standard hybridoma technology by immunizing mice with either mouse VISTA-Fc fusion proteins (mAb1) or human VISTA-expressing CHOK1 cells and boosted with human VISTA-Fc (mAb2). Isotype 1 (Iso1) and isotype 2 (Iso2) were specificities belonging to MSL-109 (anti-CMV) and AB095 (anti-tetanus toxoid) and were engineered on a human IgG1 backbone..

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An Amber Alert has cheap kamagra tablets been issued after click this a child was abducted in Pennsylvania by a man in body armor authorities say may be headed to the New York City area.Giselle Torres, age 7, is 4-feet tall, with brown eyes, brown hair, wearing a white tank top, and purple tights. She was last seen at cheap kamagra tablets 2:18 p.m. In Elkins Park, Pennsylvania, at Montgomery Avenue. (See first cheap kamagra tablets image above.)Giselle Torres was reported abducted by Juan Pablo Torres, 41, who is 5-foot-4, 160 http://gavran-hausmeister.de/impressum-datenschutz/ pounds with brown hair, brown eyes wearing body armor, a facemask, and glasses. (See second image above.)Juan Pablo Torres is to be driving a white or black Dodge Charger with unknown registration, possibly headed to the New York City area, New York State Police said.Anyone cheap kamagra tablets with information about the abduction should immediately contact the police by calling 911.Share this story by clicking on the Facebook icon below.Check back to Daily Voice for updates.

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Editorâs Note is kamagra legal in uk more (11/9/20). Ballot measures passed in the recent election included strengthening an existing ban on the use of facial recognition by police and public officials in Portland, Me., as well as other regulations of technology. This editorial provides more information on the issues with facial recognition and why jurisdictions across is kamagra legal in uk the U.S. Are attempting to control its use.Editorâs Note (11/9/20). On Tuesday the Supreme Court will hear oral arguments in the case California v.

Texas, which is kamagra legal in uk will decide whether to uphold a lower courtâs ruling that the Affordable Care Act is unconstitutional because of the individual mandate to purchase health insurance. The decision, which likely will not come for several months, could strip protections for people with preexisting conditions, including erectile dysfunction treatment. The death of Supreme Court Justice Ruth Bader Ginsburgâand President Donald Trumpâs controversial nomination of Amy Coney Barrett to fill her seatâhave ignited concerns over how a court with a six-to-three conservative majority might rule on an upcoming case on the Affordable Care Act (ACA). The Senate Judiciary Committee is scheduled to vote on is kamagra legal in uk Barrettâs confirmation this Thursday. On November 10 the court will hear California v.

Texas. That case will decide whether to uphold a lower courtâs ruling that the ACAâs individual mandate to purchase health insurance makes the entire act unconstitutionalâor to declare that the mandate is âseverableâ from the rest of it. If the ACA as a whole is struck down, 20 million people in the U.S. Would lose their insurance. Even if it is partially struck down, up to 129 million could lose protections for preexisting conditionsâincluding the more than eight million who have had erectile dysfunction treatment.

If she is confirmed before the case is heard, Barrett has given no assurances that she will vote to uphold the landmark health care law. Many legal scholars say the case for nixing the entire ACA is very weak. But even if the court severs the mandate from most of the lawâas Justice Brett Kavanaugh and others have hintedâand strikes down only parts of it, that decision could still do significant damage because the ACA is so intricately tied to the health care system, a number of experts say. Invalidating the law would âthrow the nation into economic chaos, in addition to people not having health insurance,â says Georges Benjamin, executive director of the American Public Health Association, a professional organization that promotes public health. ÂThe unintended consequences of even a small tinkering of the ACA could have enormous implications.â In 2012 the Supreme Court ruled in National Federation of Independent Business v.

Sebelius that the ACAâs individual mandate was constitutional because the penalty for not being insured could be considered a tax. But in 2017 Congress passed a tax bill that lowered the penalty to $0, beginning in January 2019. As a result, Texas and other states filed a civil suit claiming the mandate was unconstitutional in 2018. A federal judge in Texas ruled that the individual mandate was unconstitutional and nonseverable, making the entire law unconstitutionalâbut he did not overturn it. The decision was appealed and eventually made it to the Supreme Court, which is now preparing to hear the case.

A range of different outcomes is possible, according to Katie Keith, a part-time research faculty member at the Center on Health Insurance Reforms at Georgetown University and a principal at the consulting firm Keith Policy Solutions. First, the court will have to determine whether the plaintiffs have standing to challenge the mandate. ÂIf the answer is no, the case kind of goes away,â she says. Second, it must decide whether the mandate is constitutional or not. ÂReasonable minds could disagree,â she says, but the case also goes away if the mandate is found to be constitutional.

And the final question is whether the mandate is not essential to the rest of the law and can be severed from it. Keith thinks this is the most likely outcome. ÂWeâre kind of living in that world now,â she says. ÂThe mandate has been zero for two years, and the sky has not fallen.â Assuming the court determines the mandate is unconstitutional and severable from some or most of the rest of the law, it would also have to decide which parts to keep. Justices Kavanaugh and John Roberts have supported the notion of severability in a previous case.

This suggests they might vote to sever the mandate while keeping most of the ACA intact, Keith says. Nevertheless, she worries that some parts of the law could still be struck downâincluding, very importantly, the requirement that insurance companies cover preexisting conditions. That decision could affect the millions of Americans who now have, or previously had, erectile dysfunction treatment. Even though most cases of the disease are mild, there have been numerous reports of symptoms lingering for months after , as well as heart and lung damage. erectile dysfunction treatment is ânot an acute episodic diseaseâit has a long tailâ and could therefore easily qualify as a preexisting condition, says Benjamin, who was formerly secretary of the Maryland Department of Health and Mental Hygiene, a position in which he oversaw the expansion of the stateâs Medicaid program.

ÂI would assume [erectile dysfunction treatment is] a chronic condition.â But he contends that if the justices on the court cared about access to health care, âthey would care about the 20 million people who would lose health insuranceâ if the ACA were struck down. Although Barrett has been tight-lipped about how she might rule on any particular case, she has criticized the courtâs 2012 decision that upheld the ACA. ÂObviously, the future of the ACA [would be] less secure with Judge Barrett on the court, as opposed to Ginsburg,â says Tim Jost, an emeritus professor at the Washington and Lee University School of Law. ÂWe donât know much about Barrettâs views on severability. Sheâs kind of a blank slate.â The plaintiffsâ argument that the ACA is entirely dependent on the individual mandate is âabsurdâitâs factually not true,â Jost says.

ÂThe court should look at what Congress thought it was doing in 2017,â when it reduced the mandate penalty to zero. He thinks it is clear that senators and representatives did not intend to get rid of the law entirely. ÂIf what Congress thinks matters, [the court] should not invalidate the law,â he adds. But Barrett considers herself a textualist, someone who believes laws and the Constitution should be strictly interpreted with regard to the words used in them. ÂThe problem with textualists,â Jost says, âis they believe âthe law means what I say it means.ââ The ACA is an extremely complex health care law, and striking down even parts of it would be incredibly disruptiveâespecially in the midst of a kamagra.

ÂAnything can happen,â Keith says. ÂThereâs absolutely room for [the court] to strike parts of the ACA and cause some chaos.â Read more about the erectile dysfunction outbreak from Scientific American here. And read coverage from our international network of magazines here.Pfizer and BioNTech announced today that their experimental treatment against the novel erectile dysfunction is more than 90 percent effective in preventing the disease erectile dysfunction treatment, based on initial analysis of results from their large phase III trial. The two companies also report that no serious safety concerns have arisen thus far. To create the treatment, researchers genetically engineered erectile dysfunction genes, captured their blueprint, embedded that blueprint into an RNA molecule in solution and injected the solution into the bodies of trial participants.

The full method for this approach is outlined in the graphic below (light blue boxes), which originally appeared in the June 2020 issue of Scientific American. The trialâs data have not yet been submitted for peer-review publication. If the results hold up, Pfizer and BioNTech say they could manufacture large quantities of the genetically engineered treatment quickly. Credit. Jen Christiansen Read more about the erectile dysfunction outbreak from Scientific American here.

And read coverage from our international network of magazines here.Joe Biden will soon be president of the United States, and scientists the world over are breathing a collective sigh of relief. But concerns remain. Nearly half the country voted for President Donald Trump, whose actions have repeatedly undermined science and scientific institutions. Biden will have his work cut out for him in January as he takes the helm of a politically polarized nation. ÂOur long national nightmare is over,â says Alta Charo, a bioethicist at the University of Wisconsin Law School, quoting president Gerald Fordâs famous 1974 remarks about his predecessor Richard Nixonâs scandal-ridden term.

ÂI couldnât say it any better than that.â Once Biden takes office on 20 January, he will have an opportunity to reverse many policies introduced by the Trump administration that were damaging to science and public health. This includes actions on climate change, immigration and the erectile dysfunction treatment kamagra, which could claim more than a quarter of a million lives in the United States before Trump leaves office in January. Researchers are hopeful that much of the damage can be repaired. With Trump out of the picture, says Pervez Hoodbhoy, a physicist and nuclear-proliferation specialist based in Islamabad, âinstead of dog-eat-dog, maybe we will have a modicum of international cooperation, greater cheap kamagra fast adherence to laws and treaties, more civility in politics across the globe, less âfake newsâ, more smiles and less angerâ. Biden, a Democrat who served as vice-president under former president Barack Obama, has promised to ramp up US test-and-trace programmes to help bring the erectile dysfunction under control, to rejoin the Paris climate agreement to fight global warming, and to reverse travel bans and visa restrictions that have made the United States a less desirable destination for foreign researchers.

Bidenâs vice-president elect, Kamala Harris, an attorney and US senator from California, will be the first woman to achieve one of the top two offices in the country. She is also the first Black woman and the first Asian-American to be elected vice-president, in a country that has been riven by racial tensions. ÂIt is testament to the strengths and resilience of US science that it has weathered the past four years,â says James Wilsdon, a social scientist at the University of Sheffield in the UK. ÂIt can look forward now to a period of much-needed stability and support from [Bidenâs] administration.â Top priorities One of Bidenâs first orders of business will be to put a more aggressive kamagra response plan in place. On 6 November, the United States saw more than 130,000 new erectile dysfunction s recorded in a single dayâthe highest number reported anywhere across the globe since the outbreak began.

On 9 November, Biden named several prominent medical and public health figures to a erectile dysfunction task force. Trump has sought to downplay erectile dysfunction treatment while opposing state and local efforts to contain the erectile dysfunction as too costly. By contrast, Bidenâs team has committed to ramping up erectile dysfunction treatment test-and-trace programmes, working with state- and local-level officials to implement mask mandates nationwide and strengthening public-health facilities. Bidenâs team has also promised to âlisten to scienceâ. The Trump administration has repeatedly sidelined government scientists at public health agencies such as the Centers for Disease Control and Prevention and the Food and Drug Administration throughout the kamagra.

With Biden in charge, says Charo, âthere's a wide range of government agencies that now are going to finally get a chance to do their jobs properlyâ. Biden's administration will also re-open lines of communication with other countries and international organizations in its fight against the erectile dysfunction. Trump pulled the United States out of the World Health Organization earlier this year, criticizing the international agency for supporting China, where the outbreak began. ÂJoe Biden and Kamala Harris understand that no country can face our current challenges alone and hopefully will re-engage and help re-form key science-based multilateral institutions,â says Marga Gual Soler, an expert in science diplomacy and policy adviser to the European Union. Another top priority for Biden will be to reverse many of the policies impacting climate, the environment and public health put into place under Trump.

At the top of the docket is the 2015 Paris climate agreement. The United States formally withdrew from this accord on 4 November, but Biden has said he will rejoin the pact after taking office in January. Biden and Harris also campaigned on a US$2-trillion plan to boost clean energy, modernize infrastructure and curb greenhouse gas emissions. Bidenâs election holds particular significance for scientists at the US Environmental Protection Agency (EPA), which has suffered under Trumpâs efforts to roll back regulations, bolster industry influence and undermine the way science is used to craft rules to curb pollution and protect public health. ÂThe Trump administration tried to mutate the DNA of the organization,â says Dan Costa, a toxicologist who headed the agencyâs air, climate and energy research programme until January 2018 and is one of numerous veteran scientists at EPA who ultimately elected to depart during Trumpâs tenure.

It will take a while for the agency to recover, but a cloud has lifted, says Costa. ÂIâm sure people working at the EPA are breathing a sigh of relief.â A tense election Although Trump has filed lawsuits in multiple states questioning vote counts, Biden amassed enough electoral college votes to claim victory after more than four days of counting in several swing states. The record election turnout shows that "democracy beats deep in the heart of America", Biden said in a statement. "It's time to put the anger and the harsh rhetoric behind us and come together as a nation," he said. "It's time for American to unite.

And to heal." Trump has not conceded the election and has said it is "far from over". ÂIâm still nervous,â says Ali Nouri, a molecular biologist and president of the Federation of American Scientists. ÂIt's still not clear to what extent the president is going to contest the election. I think unfortunately, he has undermined some core democratic principles that we've always adhered to in this country.â This fear runs deep. During his tenure in the White House, in addition to censoring and sidelining government researchers, Trump regularly attacked political opponents as well as the media, the courts, the electoral system and other Democratic institutions.

These actions spurred some 4,000 people to sign a statement drafted by scientists that raises concerns about the state of American democracy in the run-up to the election. The closer-than-expected vote hardly served as the rebuke of Trump that many scientists were hoping for, nor did it provide a âblue waveâ across Congress that would make it easier for Biden to advance his science agenda. Democrats lost seats in the House of Representatives, although they will continue to hold a majority. And they might not wrest control of the Republican-led Senate, where the final roster will not be decided until Georgia holds a pair of run-off elections in January. Although Bidenâs election represents an imminent end to a presidency that often disregarded truth, science and evidence, many scientists fear that the movement launched by Trump will continue to haunt the United States well after he has left office.

ÂThe political defeat of Trump is enormously important. But this is not a repudiation of this larger assault on democratic civility in the United States,â says Zia Mian, a physicist and co-director of the Program in Science and Global Security at Princeton University in New Jersey. Trump has undermined core values of truth and equality, Mian says, and without those, âdemocratic debate is not possibleâ. This story was written with additional reporting by Davide Castelvecchi, Heidi Ledford, Nidhi Subbaraman and Alex Witze. This article is reproduced with permission and was first published on November 7 2020.Former Vice President Joe Biden secured the 270 electoral votes needed to capture the White House on Saturday, major news organizations projected, after election officials in a handful of swing states spent days in round-the-clock counting of millions of mail-in ballots and early votes.

The Democratâs victory came after the latest tallies showed him taking an insurmountable lead in Pennsylvania, a state both Biden and President Donald Trump had long identified as vital to their election efforts. Trump has signaled he will fight the election results in several states, filing a number of lawsuits and seeking recounts. ÂAmerica, Iâm honored that you have chosen me to lead our great country,â Biden tweeted shortly after the news organizations called the race. ÂThe work ahead of us will be hard, but I promise you this. I will be a President for all Americansâwhether you voted for me or not.â The Democratic celebration was tempered because it appeared the party would have a hard time taking back the Senate majority it lost in 2014.

If that bears out, it will likely keep Biden and Democratic lawmakers from enacting many of the plans they campaigned on, including major changes in health care. Party control of the Senate may not be determined until Januaryâthanks to what preliminary returns suggest will be runoffs for both Senate seats in Georgia. No candidate for either seat reached the required 50% threshold. Without a Democratic majority in the Senate, Biden will likely face strong Republican opposition to many of his top health agenda itemsâincluding lowering the eligibility age for Medicare to 60, expanding financial assistance for health insurance under the Affordable Care Act, and creating a âpublic optionâ government health plan. However, his administration would be a bulwark to defend the ACA against Republican attacks, although the Supreme Court case challenging the health lawâwhich will be heard next weekâpresents a major wild card for its future.

Health care was a key element of Bidenâs campaign, especially improving the federal response to the erectile dysfunction kamagra. He championed the use of face masks and blasted the Trump administration for shifting to states much of the responsibility for fighting the kamagra and helping hospitals. He was regularly mocked by the president for wearing a mask, working and campaigning from home, and not having an in-person Democratic convention. Even before the latest vote tallies were released late Saturday morning, Biden had begun moving toward setting up his administration. On Thursday his transition team unveiled a website, BuildBackBetter.com, although it was only one page.

And the former vice president held a meeting Thursday with health and economic advisers on the erectile dysfunction. In a speech to supporters in Delaware Saturday night, the president-elect pledged again to make the kamagra his top concern, saying that until erectile dysfunction treatment is under control, the country âcannot repair the economy, restore our vitality or relish lifeâs most precious moments, hugging our grandchildren, our children, our birthdays, weddings, graduations, all the moments that matter most to us.â He announced that on Monday he would name âa group of leading scientists and experts as transition advisorsâ to help develop a blueprint âbuilt on bedrock scienceâ to combat the kamagra. The electoral outcome is not the one Democrats were hoping forâor, to some extent, expecting, based on preelection polling. Andy Slavitt, who ran the Centers for Medicare &. Medicaid Services during the Obama administration, noted that frustration in a tweet Wednesday.

ÂA large disappointment is that many hoped for a significant repudiation of Trump &. His indifference to human life, human suffering, his corruption, and goal of getting rid of the ACA. No matter the final total it will be hard to make that claim,â Slavitt said. Still up in the air is how willing a Republican-led Senate will be to provide further relief to individuals, businesses and states hit hard by the kamagra, and whether they will participate in previously bipartisan efforts to curtail âsurpriseâ out-of-network medical bills and get a handle on prescription drug prices. This story was originally published by Kaiser Health News on November 7 2020.

Editorâs Note cheap kamagra tablets kamagra 100mg price in canada (11/9/20). Ballot measures passed in the recent election included strengthening an existing ban on the use of facial recognition by police and public officials in Portland, Me., as well as other regulations of technology. This editorial provides cheap kamagra tablets more information on the issues with facial recognition and why jurisdictions across the U.S. Are attempting to control its use.Editorâs Note (11/9/20). On Tuesday the Supreme Court will hear oral arguments in the case California v.

Texas, which will decide whether to uphold a lower courtâs ruling that the Affordable Care Act is unconstitutional because of the cheap kamagra tablets individual mandate to purchase health insurance. The decision, which likely will not come for several months, could strip protections for people with preexisting conditions, including erectile dysfunction treatment. The death of Supreme Court Justice Ruth Bader Ginsburgâand President Donald Trumpâs controversial nomination of Amy Coney Barrett to fill her seatâhave ignited concerns over how a court with a six-to-three conservative majority might rule on an upcoming case on the Affordable Care Act (ACA). The Senate Judiciary Committee is scheduled to vote on Barrettâs cheap kamagra tablets confirmation this Thursday. On November 10 the court will hear California v.

ÂI couldnât say it any better than that.â Once Biden takes office on 20 January, he will have an opportunity to reverse many policies introduced by the Trump administration that were damaging to science and public health. This includes actions on climate change, immigration and the erectile dysfunction treatment kamagra, which could claim more than a quarter of a million lives in the United States before Trump leaves office in January. Researchers are hopeful that much of the damage can be repaired. With Trump out of the picture, says Pervez Hoodbhoy, a physicist and nuclear-proliferation specialist based in Islamabad, âinstead of dog-eat-dog, maybe we will have a modicum of international cooperation, greater adherence to laws and treaties, more civility in politics across the globe, less âfake newsâ, more smiles and less angerâ. Biden, a Democrat who served as vice-president under former president Barack Obama, has promised to ramp up US test-and-trace programmes to help bring the erectile dysfunction under control, to rejoin the Paris climate agreement to fight global warming, and to reverse travel bans and visa restrictions that have made the United States a less desirable destination for foreign researchers.

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The U.S how to buy kamagra online. Has suffered an immense loss of life during the kamagra, with the official death count at well more than half a million to date. But the real number is likely even greater how to buy kamagra online. A new analysis has found major variations in the rates of deaths assigned to erectile dysfunction treatment among individual countiesâlikely a result of socioeconomic and political factors. Officially reported erectile dysfunction treatment deaths may not reflect the reality on the ground because of testing shortages, overwhelmed health care systems and differences in how deaths are registered.

So researchers have been examining the number of how to buy kamagra online last yearâs âexcess deathsâ (those above the expected level, based on previous years) to seek a fuller picture of the kamagraâs impact. Earlier excess death studies, conducted at the national and state level, have suggested erectile dysfunction treatment deaths have been undercounted. But there has been less research on these trends at a more granular how to buy kamagra online level. Andrew Stokes, an assistant professor in the global health department at Boston University, and his colleagues calculated excess deaths for each of more than 3,100 U.S. Counties.

To do so, they compared provisional 2020 mortality data from the National how to buy kamagra online Center for Health Statistics with predicted death rates based on previous years. The researchers then compared the proportion of excess deaths attributed to erectile dysfunction treatment on death certificates with those assigned to other causes. Their data showed that 18 how to buy kamagra online percent of excess deaths across the U.S. Last year were not assigned to erectile dysfunction treatment. And rural counties in the South and Westâparticularly in the East South Central regionâhad some of the highest rates of excess deaths assigned to non-erectile dysfunction treatment causes.

The findings add to the evidence that a significant number of erectile dysfunction treatment deaths may have been undercounted how to buy kamagra online. They could also reflect an increase in other types of deaths caused by the kamagraâs social and economic effects, often among disadvantaged populations. ÂOne consequence of underreporting of erectile dysfunction treatment deaths in a community is that it may create the how to buy kamagra online appearance that the kamagra did not affect that community and that the nonpharmaceutical interventions, vaccination and other preventive measures are not necessary,â Stokes warns. ÂPeople act on information. And if theyâre not seeing erectile dysfunction treatment mortality in their communities, theyâre less likely to act.â Credit.

Calvin A how to buy kamagra online. Ackley and Andrew C. Stokes (map) how to buy kamagra online. Restyled by Amanda MontaÃ±ez. Source.

ÂCounty-Level Estimates of Excess Mortality Associated with erectile dysfunction treatment in the United States,â by how to buy kamagra online Calvin A. Ackley et al. Preprint posted May 5, 2021, to www.medrxiv.org/content/10.1101/2021.04.23.21255564v2 how to buy kamagra online The findings were reported this spring in a preprint study that has not yet been peer-reviewed. The chart below reveals the counties that had the highest ratios of non-erectile dysfunction treatment excess deaths to those assigned to erectile dysfunction treatment. Credit.

Amanda MontaÃ±ez how to buy kamagra online. Source. ÂCounty-Level Estimates of Excess Mortality Associated with erectile dysfunction treatment in the United States,â by Calvin A. Ackley et al how to buy kamagra online. Preprint posted May 5, 2021, to www.medrxiv.org/content/10.1101/2021.04.23.21255564v2 The county-level data also revealed trends that were not apparent from state-level figures.

Take Florida how to buy kamagra online. The stateâs official numbers are often held up as being no worse than average in terms of erectile dysfunction treatment deaths, despite its governor taking a relatively relaxed approach to kamagra-related restrictions on activities. But Stokesâs team observed a lot more excess deaths not attributed to erectile dysfunction treatment in some of Floridaâs rural counties, compared with more urban ones. Yet rural counties were not the only places that saw high numbers of excess how to buy kamagra online deaths. Three of the five American counties with the most excess deaths overall were in New York City, which was the epicenter of the kamagra last spring.

Credit how to buy kamagra online. Amanda MontaÃ±ez. Source. ÂCounty-Level Estimates of Excess Mortality Associated with erectile dysfunction treatment in the United States,â how to buy kamagra online by Calvin A. Ackley et al.

Preprint posted May 5, 2021, to www.medrxiv.org/content/10.1101/2021.04.23.21255564v2 Interestingly, in some counties in New Englandâs metropolitan areas, reported erectile dysfunction treatment deaths actually exceeded excess deaths in 2020, suggesting these regions had reductions in how to buy kamagra online other causes of death. For example, far fewer people may have died from the flu (which virtually disappeared during the kamagra)âas a result of many taking public health precautions and working from home. Credit. Amanda MontaÃ±ez how to buy kamagra online. Source.

ÂCounty-Level Estimates of Excess how to buy kamagra online Mortality Associated with erectile dysfunction treatment in the United States,â by Calvin A. Ackley et al. Preprint posted May 5, 2021, to www.medrxiv.org/content/10.1101/2021.04.23.21255564v2 There are a number of possible reasons for county-level gaps between excess deaths and reported erectile dysfunction treatment deaths. One is the lack of available testing early in the kamagra, when many health care systems were overwhelmed how to buy kamagra online. And erectile dysfunction treatment was less likely to be listed on death certificates without a positive test.

Many people who died of the illness were in a nursing home, where they could not always get a test how to buy kamagra online. Others died at home because they could not get to a hospital in time or were afraid to go to one. Many deaths attributed to causes such as Alzheimerâs disease or a heart attack may have actually resulted from erectile dysfunction treatment. Another possibility is that differences in how deaths are certifiedâand possibly political biasâplayed a role how to buy kamagra online in the variation seen among counties. Unlike urban areas, where doctors or medical examiners often certify deaths, in many rural counties, this job is done by coroners, coroner-sheriffs or other elected or appointed officials who do not have a medical background.

ÂIn Texas, for example, while most metro counties have their own medical examiner for death investigation, rural areas frequently rely on justices of the peace,â Stokes says. These justices âoften lack medical training and resources for postmortem testing and may have how to buy kamagra online their own political biases that affect death certification.â Rural U.S. Areas also tend to support conservative politicians, many of whom questioned or denied the severity of the election-year kamagra threat. Stokes and his team hypothesize that the political views of these officials may have influenced their decisions not how to buy kamagra online to list erectile dysfunction treatment as the primary cause of death. The gap between erectile dysfunction treatment deaths and excess deaths could also reflect an increase in other causes of mortality that were indirectly affected by the kamagra.

These could include acute illnesses, such as a heart attack or stroke, that were left untreated because hospitals were overwhelmed and people were avoiding care. Chronic illnesses, how to buy kamagra online such as cancer or diabetes, that were not controlled because the kamagraâs economic impacts made it harder to access and afford treatment. And an increase in social isolation that may have led to deaths from suicide or a drug overdose. The study did how to buy kamagra online not try to determine the proportion of a given countyâs excess deaths that may have resulted directly from erectile dysfunction treatment versus related factors such as those described above, because detailed county-level cause-of-death data have not yet been released. The national mortality information in these analyses was provisional and may be subject to change when final data are released.

The data set also included independent cities that are not part of a larger county. The research âprovides further additional evidence that the burden of the kamagra is higher than the reported numbers and some places in particular might have a larger number of how to buy kamagra online unaccounted for erectile dysfunction treatment deaths,â says Daniel Weinberger, an associate professor of epidemiology at the Yale School of Public Health, who co-authored a previous study of excess deaths but was not involved in the new analysis. He notes, however, that âmore work is needed to untangle the contribution of how much are direct viral deaths and how much are unrelated changes due to disruptions in health care.â Kirsten Bibbins-Domingo, chair of the department of epidemiology and biostatistics at the University of California, San Francisco, emphasizes the importance of understanding these trends. ÂWe are how to buy kamagra online sort of numb to the number of people who have died of erectile dysfunction treatment,â says Bibbins-Domingo, who was not involved in the new study. ÂThis phenomenally detailed county-level analysis tells us that thereâs a lot of variation, and that variation falls deeply along racial and class lines.

In places that have lower income, in places with more minority populations, in places with worse health status to begin with, we see that the impacts of the kamagra are likely far greater than even the stark disparities in the kamagra have suggested thus far.â Stokes and his colleagues recently published another study on excess deaths among clusters of counties in PLOS Medicine. This work found that low-income communities, as well as those with a high how to buy kamagra online proportion of Black people, had greater numbers of excess deaths that were not assigned to erectile dysfunction treatment. Many people of color work in essential jobs that have put them at a greater risk of exposure to the novel erectile dysfunction, and many of them have had poorer health as a result of decades of income inequality and structural racism. ÂThe whole overarching picture of this research really needs to focus on the underlying systems that contributed to vulnerability during the kamagra,â says Dielle Lundberg, a research fellow at Boston University, who was a co-author of both the new preprint and the how to buy kamagra online PLOS Medicine study. ÂIf we want to improve public health, we need to address these underlying systems.â Stokes worries that an undercounting of erectile dysfunction treatment deaths may be fueling treatment hesitancy in many areas.

His team has looked at the proportion of excess deaths that were not attributed to erectile dysfunction treatment and how it relates to treatment uptake and hesitancy. ÂThere is a clear and compelling pattern whereby places with the most treatment hesitancy and how to buy kamagra online the lowest treatment uptake are the same communities that had the most excess deaths not assigned to erectile dysfunction treatment,â he says. ÂIf we rectify those undercounts, the question is. Could that have immediate impact on treatment how to buy kamagra online uptake in communities?. Â Perhaps the biggest takeaway from this research is that U.S.

Counties need more systematic ways of certifying deaths, Bibbins-Domingo says. ÂItâs not very sexy to say that we need to be able to collect better data,â she notes how to buy kamagra online. ÂBut when data drives policyâand data drives policy that is aimed at addressing inequitiesâgood data collection is absolutely critical.â IF YOU NEED HELPIf you or someone you know is struggling or having thoughts of suicide, help is available. Call the National Suicide Prevention Lifeline at 1-800-273-8255 (TALK), use the online Lifeline Chat or contact the Crisis Text Line by texting TALK to 741741..

The U.S cheap kamagra tablets https://www.europlanet-society.org/online-pharmacy-cialis/. Has suffered an immense loss of life during the kamagra, with the official death count at well more than half a million to date. But the real number is likely cheap kamagra tablets even greater. A new analysis has found major variations in the rates of deaths assigned to erectile dysfunction treatment among individual countiesâlikely a result of socioeconomic and political factors.

Officially reported erectile dysfunction treatment deaths may not reflect the reality on the ground because of testing shortages, overwhelmed health care systems and differences in how deaths are registered. So researchers have been cheap kamagra tablets examining the number of last yearâs âexcess deathsâ (those above the expected level, based on previous years) to seek a fuller picture of the kamagraâs impact. Earlier excess death studies, conducted at the national and state level, have suggested erectile dysfunction treatment deaths have been undercounted. But there has been less research on these cheap kamagra tablets trends at a more granular level.

Andrew Stokes, an assistant professor in the global health department at Boston University, and his colleagues calculated excess deaths for each of more than 3,100 U.S. Counties. To do so, they compared provisional 2020 mortality data from the National Center for Health Statistics with predicted death rates based on cheap kamagra tablets previous years. The researchers then compared the proportion of excess deaths attributed to erectile dysfunction treatment on death certificates with those assigned to other causes.

Their data showed that 18 cheap kamagra tablets percent of excess deaths across the U.S. Last year were not assigned to erectile dysfunction treatment. And rural counties in the South and Westâparticularly in the East South Central regionâhad some of the highest rates of excess deaths assigned to non-erectile dysfunction treatment causes. The findings cheap kamagra tablets add to the evidence that a significant number of erectile dysfunction treatment deaths may have been undercounted.

They could also reflect an increase in other types of deaths caused by the kamagraâs social and economic effects, often among disadvantaged populations. ÂOne consequence of underreporting of erectile dysfunction treatment deaths in a community is that it may create the appearance that the kamagra did not affect cheap kamagra tablets that community and that the nonpharmaceutical interventions, vaccination and other preventive measures are not necessary,â Stokes warns. ÂPeople act on information. And if theyâre not seeing erectile dysfunction treatment mortality in their communities, theyâre less likely to act.â Credit.

Calvin A cheap kamagra tablets. Ackley and Andrew C. Stokes (map) cheap kamagra tablets. Restyled by Amanda MontaÃ±ez.

Source. ÂCounty-Level Estimates of Excess Mortality Associated cheap kamagra tablets with erectile dysfunction treatment in the United States,â by Calvin A. Ackley et al. Preprint posted May 5, 2021, to cheap kamagra tablets www.medrxiv.org/content/10.1101/2021.04.23.21255564v2 The findings were reported this spring in a preprint study that has not yet been peer-reviewed.

The chart below reveals the counties that had the highest ratios of non-erectile dysfunction treatment excess deaths to those assigned to erectile dysfunction treatment. Credit. Amanda MontaÃ±ez cheap kamagra tablets. Source.

ÂCounty-Level Estimates of Excess Mortality Associated with erectile dysfunction treatment in the United States,â by Calvin A. Ackley et cheap kamagra tablets al. Preprint posted May 5, 2021, to www.medrxiv.org/content/10.1101/2021.04.23.21255564v2 The county-level data also revealed trends that were not apparent from state-level figures. Take Florida cheap kamagra tablets.

The stateâs official numbers are often held up as being no worse than average in terms of erectile dysfunction treatment deaths, despite its governor taking a relatively relaxed approach to kamagra-related restrictions on activities. But Stokesâs team observed a lot more excess deaths not attributed to erectile dysfunction treatment in some of Floridaâs rural counties, compared with more urban ones. Yet rural counties cheap kamagra tablets were not the only places that saw high numbers of excess deaths. Three of the five American counties with the most excess deaths overall were in New York City, which was the epicenter of the kamagra last spring.

Credit cheap kamagra tablets. Amanda MontaÃ±ez. Source. ÂCounty-Level Estimates of Excess Mortality Associated with erectile dysfunction treatment in the United States,â by Calvin cheap kamagra tablets A.

Ackley et al. Preprint posted May 5, 2021, to www.medrxiv.org/content/10.1101/2021.04.23.21255564v2 Interestingly, in some counties in New Englandâs metropolitan areas, reported erectile dysfunction treatment deaths actually exceeded excess deaths in 2020, suggesting these cheap kamagra tablets regions had reductions in other causes of death. For example, far fewer people may have died from the flu (which virtually disappeared during the kamagra)âas a result of many taking public health precautions and working from home. Credit.

Amanda MontaÃ±ez cheap kamagra tablets. Source. ÂCounty-Level Estimates of Excess Mortality Associated with erectile dysfunction treatment in cheap kamagra tablets the United States,â by Calvin A. Ackley et al.

Preprint posted May 5, 2021, to www.medrxiv.org/content/10.1101/2021.04.23.21255564v2 There are a number of possible reasons for county-level gaps between excess deaths and reported erectile dysfunction treatment deaths. One is the lack of cheap kamagra tablets available testing early in the kamagra, when many health care systems were overwhelmed. And erectile dysfunction treatment was less likely to be listed on death certificates without a positive test. Many people who died of cheap kamagra tablets the illness were in a nursing home, where they could not always get a test.

Others died at home because they could not get to a hospital in time or were afraid to go to one. Many deaths attributed to causes such as Alzheimerâs disease or a heart attack may have actually resulted from erectile dysfunction treatment. Another possibility is that differences in how deaths cheap kamagra tablets are certifiedâand possibly political biasâplayed a role in the variation seen among counties. Unlike urban areas, where doctors or medical examiners often certify deaths, in many rural counties, this job is done by coroners, coroner-sheriffs or other elected or appointed officials who do not have a medical background.

ÂIn Texas, for example, while most metro counties have their own medical examiner for death investigation, rural areas frequently rely on justices of the peace,â Stokes says. These justices âoften lack medical training cheap kamagra tablets and resources for postmortem testing and may have their own political biases that affect death certification.â Rural U.S. Areas also tend to support conservative politicians, many of whom questioned or denied the severity of the election-year kamagra threat. Stokes and his team hypothesize that the political views of these officials may have influenced their decisions cheap kamagra tablets not to list erectile dysfunction treatment as the primary cause of death.

The gap between erectile dysfunction treatment deaths and excess deaths could also reflect an increase in other causes of mortality that were indirectly affected by the kamagra. These could include acute illnesses, such as a heart attack or stroke, that were left untreated because hospitals were overwhelmed and people were avoiding care. Chronic illnesses, such as cancer or diabetes, that were not controlled because the kamagraâs economic impacts made it harder to access and afford treatment cheap kamagra tablets. And an increase in social isolation that may have led to deaths from suicide or a drug overdose.

The study did not try to determine the proportion of a given countyâs excess deaths that may have resulted directly from erectile dysfunction treatment versus related factors such as those described above, because cheap kamagra tablets detailed county-level cause-of-death data have not yet been released. The national mortality information in these analyses was provisional and may be subject to change when final data are released. The data set also included independent cities that are not part of a larger county. The research âprovides further additional evidence that the burden of the kamagra is higher than the reported numbers and some places in particular might have a larger number of unaccounted for erectile dysfunction treatment deaths,â says Daniel Weinberger, an associate professor of epidemiology at the Yale School of cheap kamagra tablets Public Health, who co-authored a previous study of excess deaths but was not involved in the new analysis.

He notes, however, that âmore work is needed to untangle the contribution of how much are direct viral deaths and how much are unrelated changes due to disruptions in health care.â Kirsten Bibbins-Domingo, chair of the department of epidemiology and biostatistics at the University of California, San Francisco, emphasizes the importance of understanding these trends. ÂWe are cheap kamagra tablets sort of numb to the number of people who have died of erectile dysfunction treatment,â says Bibbins-Domingo, who was not involved in the new study. ÂThis phenomenally detailed county-level analysis tells us that thereâs a lot of variation, and that variation falls deeply along racial and class lines. In places that have lower income, in places with more minority populations, in places with worse health status to begin with, we see that the impacts of the kamagra are likely far greater than even the stark disparities in the kamagra have suggested thus far.â Stokes and his colleagues recently published another study on excess deaths among clusters of counties in PLOS Medicine.

This work found that low-income communities, as well as those with a high proportion of Black people, had greater numbers of excess deaths that were not assigned to erectile dysfunction treatment cheap kamagra tablets. Many people of color work in essential jobs that have put them at a greater risk of exposure to the novel erectile dysfunction, and many of them have had poorer health as a result of decades of income inequality and structural racism. ÂThe whole overarching picture of this research really needs to focus on cheap kamagra tablets the underlying systems that contributed to vulnerability during the kamagra,â says Dielle Lundberg, a research fellow at Boston University, who was a co-author of both the new preprint and the PLOS Medicine study. ÂIf we want to improve public health, we need to address these underlying systems.â Stokes worries that an undercounting of erectile dysfunction treatment deaths may be fueling treatment hesitancy in many areas.

His team has looked at the proportion of excess deaths that were not attributed to erectile dysfunction treatment and how it relates to treatment uptake and hesitancy. ÂThere is a clear and compelling pattern whereby places with the most treatment hesitancy and the lowest treatment uptake are the same communities that had the most excess deaths not assigned cheap kamagra tablets to erectile dysfunction treatment,â he says. ÂIf we rectify those undercounts, the question is. Could that have immediate impact on treatment uptake in cheap kamagra tablets communities?.

Â Perhaps the biggest takeaway from this research is that U.S. Counties need more systematic ways of certifying deaths, Bibbins-Domingo says. ÂItâs not very sexy to say that we need to be able to collect better data,â she notes. ÂBut when data drives policyâand data drives policy that is aimed at addressing inequitiesâgood data collection is absolutely critical.â IF YOU NEED HELPIf you or someone you know is struggling or having thoughts of suicide, help is available.

Call the National Suicide Prevention Lifeline at 1-800-273-8255 (TALK), use the online Lifeline Chat or contact the Crisis Text Line by texting TALK to 741741..

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Medicaid Services (CMS) Innovation Center. The model is testing the impact of systematically identifying and addressing health-related social kamagra oral jelly 50mg needs among Medicare and Medicaid beneficiaries. To help providers administer the screening tool, Mathematica developed, on CMSâs behalf, a set of instructions for users called âA Guide to Using the Accountable Health Communities Health-Related Social Needs Screening Tool.

Promising Practices and Key Insights.âThe Accountable kamagra oral jelly 50mg Health Communities Health-Related Social Needs Screening Tool enables users to quickly assess patientsâ social needs from five domains that CMS determined as core needs (living situation, food, transportation, utilities, and safety) and eight supplemental domains (financial strain, employment, family and community support, education, physical activity, substance use, mental health, and disabilities). The screening tool is appropriate for use in a wide range of clinical settings, including primary care practices, emergency departments, labor and delivery units, inpatient psychiatric units, behavioral health clinics, and other places where people access clinical care. The tool is available in three versions.

(1) a standard self-administered version, kamagra oral jelly 50mg (2) a proxy version with questions adapted to enable someone to answer on behalf of the patient, and (3) a multiuse version that includes language for a proxy and for patients answering for themselves.After quickly identifying social needs using the screening tool, health care or social service providers can then connect patients with community resources to address the patientsâ unmet needs.Implementing universal health-related social needs screening in clinical settings requires planning, which includes aligning priorities, training staff, and developing customized screening protocols. In light of this, the guide also includes lessons based on the experiences of organizations participating in the Accountable Health Communities Model. The strategies shared in the guide are meant to inform effective universal screening in a wide range of clinical settings.Promising practices for universal screening described in the guide Cultivate staff buy-in Tailor staffing models to site features Provide dedicated training kamagra oral jelly 50mg on screening Use customized scripts to engage patients in screening Consider the timing, location, and process for screening to maximize patientsâ participation Anticipate population-specific needs Train staff to manage privacy and address safety concerns Institute continuous quality improvement Prepare staff to respond to common questionsFor more information on the AHC Screening Guide, please contact Lee-Lee Ellis and Rachel Kogan.As more districts commit to offering in-person learning for most or all of their students, many are grappling with the costs and benefits of conducting routine erectile dysfunction treatment testing as part of their comprehensive prevention approach, as recommended by the CDCâs recent guidance for K-12 schools.

School districts now have access to tremendous resources to stand up testing in K-12 settings, including the recent allocation of $10 billion for school testing in the American Rescue Plan, as well as implementation guidance including The Rockefeller Foundationâs K-12 National Testing Action Program (NTAP) and Playbook for Educators and Leaders. However, many kamagra oral jelly 50mg may need more data on the impact of testing to make an informed decision.As many school districts work to make routine testing a reality, Mathematica, The Rockefeller Foundation, and the Duke Margolis Health Policy Center partnered to create an interactive online dashboard that allows users to explore the benefits and drawbacks of routine testing in their schools to guide decisions about which testing strategies to implement.You can view the dashboard here. For more information, please contact Divya Vohra or John Hotchkiss.

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Mounting real-world evidence shows universal screening for health-related social cheap kamagra tablets needs in routine clinical care offers a standardized way for health care providers to identify needs, tailor care, and help patients resolve these needs with referrals to community resources i thought about this. Yet screening for patientsâ social needs can seem like a daunting task for clinical providers. One strategy for providers is to first identify patientsâ social needs by administering a screening tool such as the one developed for the Accountable Health Communities Model, a nationwide initiative funded by the Centers for Medicare cheap kamagra tablets &.

Medicaid Services (CMS) Innovation Center. The model cheap kamagra tablets is testing the impact of systematically identifying and addressing health-related social needs among Medicare and Medicaid beneficiaries. To help providers administer the screening tool, Mathematica developed, on CMSâs behalf, a set of instructions for users called âA Guide to Using the Accountable Health Communities Health-Related Social Needs Screening Tool.

Promising Practices and Key Insights.âThe Accountable Health Communities Health-Related Social Needs Screening Tool enables users to quickly assess patientsâ social needs from five domains that CMS determined as core needs (living situation, food, transportation, utilities, and safety) and eight supplemental domains (financial strain, employment, family and community support, cheap kamagra tablets education, physical activity, substance use, mental health, and disabilities). The screening tool is appropriate for use in a wide range of clinical settings, including primary care practices, emergency departments, labor and delivery units, inpatient psychiatric units, behavioral health clinics, and other places where people access clinical care. The tool is available buy kamagra jelly in three versions.

(1) a standard self-administered cheap kamagra tablets version, (2) a proxy version with questions adapted to enable someone to answer on behalf of the patient, and (3) a multiuse version that includes language for a proxy and for patients answering for themselves.After quickly identifying social needs using the screening tool, health care or social service providers can then connect patients with community resources to address the patientsâ unmet needs.Implementing universal health-related social needs screening in clinical settings requires planning, which includes aligning priorities, training staff, and developing customized screening protocols. In light of this, the guide also includes lessons based on the experiences of organizations participating in the Accountable Health Communities Model. The strategies shared in cheap kamagra tablets the guide are meant to inform effective universal screening in a wide range of clinical settings.Promising practices for universal screening described in the guide Cultivate staff buy-in Tailor staffing models to site features Provide dedicated training on screening Use customized scripts to engage patients in screening Consider the timing, location, and process for screening to maximize patientsâ participation Anticipate population-specific needs Train staff to manage privacy and address safety concerns Institute continuous quality improvement Prepare staff to respond to common questionsFor more information on the AHC Screening Guide, please contact Lee-Lee Ellis and Rachel Kogan.As more districts commit to offering in-person learning for most or all of their students, many are grappling with the costs and benefits of conducting routine erectile dysfunction treatment testing as part of their comprehensive prevention approach, as recommended by the CDCâs recent guidance for K-12 schools.

School districts now have access to tremendous resources to stand up testing in K-12 settings, including the recent allocation of $10 billion for school testing in the American Rescue Plan, as well as implementation guidance including The Rockefeller Foundationâs K-12 National Testing Action Program (NTAP) and Playbook for Educators and Leaders. However, many may need more data on the impact of testing to make an cheap kamagra tablets informed decision.As many school districts work to make routine testing a reality, Mathematica, The Rockefeller Foundation, and the Duke Margolis Health Policy Center partnered to create an interactive online dashboard that allows users to explore the benefits and drawbacks of routine testing in their schools to guide decisions about which testing strategies to implement.You can view the dashboard here. For more information, please contact Divya Vohra or John Hotchkiss.

Click here for a description of agent-based models..